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Related Experiment Video

Updated: Jun 4, 2025

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Published on: May 27, 2021

EMT-Induced Morphological Variations on Living Cell Membrane Surface.

Hiroki Ida^1,2,3,4,5, Noriko Taira⁵, Yuji Nashimoto^2,6

¹Department of Electrical Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi 464-8601, Japan.

Analytical Chemistry

|January 2, 2025

Summary

Scanning ion conductance microscopy (SICM) revealed that epithelial-mesenchymal transition (EMT) reduces cell surface ruffles. SICM surface measurements can track EMT progression by monitoring these nanoscale membrane changes.

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Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Epithelial-mesenchymal transition (EMT) is a critical cellular process where epithelial cells gain a mesenchymal phenotype.
Understanding EMT-induced cellular changes is vital for developmental biology and disease research.
Noninvasive methods for observing EMT at the nanoscale are needed.

Purpose of the Study:

To investigate nanoscale membrane variations during EMT using scanning ion conductance microscopy (SICM).
To determine if SICM can serve as an indicator for assessing EMT progression.

Main Methods:

Utilized scanning ion conductance microscopy (SICM) for noninvasive nanoscale visualization of living cells.
Performed time-lapse imaging to observe dynamic changes during EMT induction.

Main Results:

The number and size of cell surface ruffles decreased as EMT progressed.
Overall cell shape changes preceded nanoscale morphological variations.
Small ruffles exhibited active movement even after EMT induction.

Conclusions:

SICM enables noninvasive nanoscale observation of EMT-induced membrane changes.
Decreased cell surface ruffles and their dynamics are potential biomarkers for EMT progression.
SICM-based surface shape measurements offer a valuable tool for assessing EMT.