Sustainable Formation of Gold Nanoparticle-Decorated Amyloid Fibrils for the Development of Functional Hybrid...

Amyloid fibrils have recently emerged as promising building blocks for functional materials due to their exceptional physicochemical stability and adaptable properties. These protein-based structures can be functionalized to create hybrid materials with a diverse range of applications. Here we report a simple eco-friendly protocol for generating amyloid fibrils from hen egg white lysozyme decorated with gold nanoparticles that can self-assemble in a hydrogel. Reactive oligomeric species act as reducing agents, enabling the efficient and simple formation of small gold nanoparticles without the need of harsh reagents. Furthermore, the protein molecules template the formation of gold nanoparticles, which are stabilized at regular intervals along the fibril axis, preserving gold nanoparticle properties at a macroscopic scale. As an illustration of potential application, we show that the gold nanoparticle functionalized hydrogel can be employed to sense and quantify creatinine using fluorescence detection. These findings reinforce the growing interest in utilizing proteins as foundational elements for functional biomaterials due to their high biocompatibility, availability, and the ability to finely tune supramolecular assemblies.