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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Aggregation-Induced Emission Luminogens Realizing High-Contrast Bioimaging.

Wen-Jin Wang¹, Zhuo-Yang Xin¹, Xuxian Su²

¹Clinical Translational Research Center of Aggregation-Induced Emission, The Second Affiliated Hospital, School of Medicine, School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen (CUHK-Shenzhen), Shenzhen, Guangdong 518172, China.

|January 2, 2025

Summary

This summary is machine-generated.

Aggregation-induced emission luminogens (AIEgens) revolutionize biomedical imaging by overcoming traditional probe limitations. These molecules enhance luminescence upon aggregation, enabling superior high-contrast imaging for disease detection and personalized medicine.

Keywords:

Aggregation-Induced Emission (AIE)High-Contrast Imaging Molecular Design Strategies Near-Infrared (NIR-II) Imaging Personalized Medicine Responsive Imaging Theranostics Time-Resolved (TR) Imaging

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Area of Science:

Biomedical Imaging
Materials Science
Organic Chemistry

Background:

Traditional fluorescent probes suffer from aggregation-caused quenching, limiting imaging contrast.
Aggregation-induced emission luminogens (AIEgens) offer a novel solution by enhancing luminescence upon aggregation.

Purpose of the Study:

To review the molecular mechanisms of aggregation-induced emission (AIE).
To highlight the design strategies for AIEgens to achieve high luminescence and contrast.
To explore the applications of AIEgens in advanced biomedical imaging.

Main Methods:

Review of molecular mechanisms behind AIE.
Analysis of molecular design principles for AIEgens.
Compilation of key applications in biomedical imaging.

Main Results:

AIEgens exhibit enhanced luminescence in aggregated states, overcoming quenching effects.
Strategic molecular design enables superior imaging contrast for distinguishing healthy and diseased tissues.
AIEgens show promise in time-resolved imaging, NIR-II imaging, and cue-responsive imaging.

Conclusions:

AIE technology represents a paradigm shift in biophotonics for molecular-level biological system analysis.
AIEgens are poised to transform healthcare, from early disease detection to targeted therapies and personalized medicine.
The development of AIEgens facilitates the visualization of biological processes, advancing diagnostics and treatments.