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Updated: Jun 4, 2025

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
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From Self-Assembly to Drug Delivery: Understanding and Exploring Protein Fibrils.

Anu Jain1, Manu Lopus2, Nand Kishore1

  • 1Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.

Langmuir : the ACS Journal of Surfaces and Colloids
|January 2, 2025
PubMed
Summary
This summary is machine-generated.

Pluronics accelerate lysozyme fibrillation, forming aggregates. These protein aggregates can encapsulate drugs like 5-fluorouracil, enhancing their delivery and leading to cell death in cancer treatment.

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Area of Science:

  • Biomedicine and Pharmaceutical Sciences
  • Protein Misfolding and Aggregation
  • Biophysical Chemistry

Background:

  • Protein misfolding and aggregation into amyloid fibrils cause amyloidosis, a debilitating disease.
  • Understanding these processes is critical for biomedicine and pharmaceutical development.
  • Pluronics are polymers with potential applications in protein stabilization and drug delivery.

Purpose of the Study:

  • To investigate the mechanism of lysozyme fibrillation in the presence of pluronics (F68 and F127).
  • To explore the potential of pluronic-induced protein aggregates as drug delivery vehicles.
  • To evaluate the efficacy of drug-loaded aggregates against malignant cells.

Main Methods:

  • Calorimetric and spectroscopic techniques to study protein aggregation.
  • Transmission Electron Microscopy (TEM) and Thioflavin T (ThT) binding assays for fibril characterization.
  • Drug adsorption studies using 5-fluorouracil and cytarabine on protein fibrils.

Main Results:

  • Pluronics (F68, F127) were found to accelerate lysozyme fibrillation, forming amorphous aggregates.
  • Drug interaction studies revealed differential adsorption: 5-fluorouracil showed significant adsorption, while cytarabine exhibited weak adsorption.
  • The combination of lysozyme-fibrils, Pluronic F127, and 5-fluorouracil demonstrated lethality towards malignant cells.

Conclusions:

  • Pluronics modulate lysozyme fibrillation, leading to the formation of drug-carrying aggregates.
  • Protein aggregates show potential as carriers for 5-fluorouracil, with significant adsorption and therapeutic efficacy.
  • Further research into protein aggregate-based drug encapsulation and delivery is warranted.