Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.4K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.4K
Abnormal Proliferation02:23

Abnormal Proliferation

4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Deficiency of USP20 Alleviates Pressure Overload-Induced Cardiac Hypertrophy via the NF-κB Signaling Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Integrative machine learning and multi-omics analysis reveals ATIC as a promoter of hepatocellular carcinoma progression.

Scientific reports·2026
Same author

Effect of two double-stranded RNA viruses on the virulence of the phytopathogenic fungus Fusarium oxysporum.

Archives of virology·2026
Same author

Photocatalytic [4 + 1] Annulation of Vinyl Aziridines with Alkyl Halides.

Organic letters·2026
Same author

Programmable Semi-Interpenetrating Living Materials With Robust Stability for Versatile Bioremediation and Biotherapeutics.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Associations Between Controlling Nutritional Status and Allostatic Load With Heart Failure Across Different Depressive States: A Cross-Sectional Study Using NHANES 2005-2018 Data.

Reviews in cardiovascular medicine·2026
Same journal

Diosgenin alleviates radiation nephropathy by suppressing renal mTORC1 signalling with concomitant effects on the gut and liver.

Cellular signalling·2026
Same journal

Glycine induces MafG to regulate glutathione metabolism, inhibit chondrocyte ferroptosis, and upregulate plectin to improve osteoarthritis.

Cellular signalling·2026
Same journal

SHC4 suppresses ferroptosis and promotes sorafenib resistance in hepatocellular carcinoma by disrupting the interaction between NCOA4 and FTH1.

Cellular signalling·2026
Same journal

Post-translational modifications in triple-negative breast cancer: research status and translation challenges.

Cellular signalling·2026
Same journal

PDK4-dependent lactate production and lactylation promote renal calcium oxalate crystal-induced EMT and mitochondrial dysfunction via the TGF-β/SMAD3/GPX4 axis.

Cellular signalling·2026
Same journal

O-GlcNAcylation-mediated glycolytic reprogramming of CD4<sup>+</sup> T cells contributes to type H vessel impairment in diabetic osteoporosis.

Cellular signalling·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

17.8K

USP27 promotes glycolysis and hepatocellular carcinoma progression by stabilizing PFKFB3 through deubiquitination.

Longhui Xie1, Dekun Song2, Zhengsheng Ouyang3

  • 1Department of Hepatobiliary Pancreatic Spleen Surgery, The Central Hospital of Yongzhou, Yongzhou 425000, PR China.

Cellular Signalling
|January 2, 2025
PubMed
Summary
This summary is machine-generated.

This study reveals that USP27 promotes hepatocellular carcinoma (HCC) growth by enhancing glycolysis. Targeting the CTCF/USP27/PFKFB3 pathway offers a potential new strategy for treating HCC and reducing metastasis.

Keywords:
CTCFDeubiquitinationGlycolysisHCCPFKFB3USP27

More Related Videos

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.0K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.5K

Related Experiment Videos

Last Updated: Jun 4, 2025

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

17.8K
SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
08:29

SUMO-Binding Entities SUBEs as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Published on: November 1, 2019

7.0K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.5K

Area of Science:

  • Oncology
  • Molecular Biology
  • Metabolic Pathways

Background:

  • Hepatocellular carcinoma (HCC) presents a poor prognosis due to frequent metastasis and recurrence.
  • Metabolic reprogramming, particularly increased glycolysis, is a hallmark of cancer progression.

Purpose of the Study:

  • To identify key regulators of glycolysis in HCC.
  • To elucidate the molecular mechanisms linking transcriptional regulation and metabolic reprogramming in HCC.

Main Methods:

  • Investigated the role of USP27 in HCC glycolysis.
  • Utilized gene knockdown and overexpression in HCC cell lines.
  • Performed in vivo studies to assess tumor growth and metastasis.
  • Analyzed the interaction between CTCF, USP27, and PFKFB3.

Main Results:

  • USP27 was identified as a crucial regulator of glycolysis in HCC.
  • USP27 stabilizes PFKFB3 via deubiquitination, increasing glycolytic activity.
  • CTCF directly upregulates USP27 expression by binding to its promoter.
  • Knockdown of USP27 or CTCF inhibited HCC cell glycolysis and proliferation.
  • USP27 knockdown suppressed HCC growth and metastasis in vivo.

Conclusions:

  • The CTCF/USP27/PFKFB3 axis represents a novel mechanism driving HCC progression through enhanced glycolysis.
  • Targeting USP27-mediated metabolic pathways presents a promising therapeutic strategy for HCC treatment.