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Updated: Jul 13, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
[Improving the application of metagenomic next-generation sequencing for pathogen diagnosis in infections related to
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China Tianjin Institutes of Health Science, Tianjin 301600, China.
Abstract:
Patients with hematologic diseases are prone to infections, and infection-related mortality is high in this population. Accurate diagnosis of infectious pathogens is crucial; however, conventional microbiological testing often fails to meet clinical needs, presenting a significant challenge in clinical practice. Metagenomic next-generation sequencing (mNGS), with its high sensitivity, broad coverage, and short turnaround time, has been widely adopted in diagnosing infectious diseases. This article focuses on the application of mNGS technology in diagnosing infectious pathogens in patients with hematologic diseases. It analyzes the challenges encountered in clinical practice and explores future trends in the field. This work aims to provide clinicians with an in-depth understanding of the value and limitations of mNGS in diagnosing infections in hematologic patients, and to promote its rational application.
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