Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered CCR2 positive macrophages coordinate immunoregulation with neural regeneration and matrix remodeling after spinal cord injury.

Theranostics·2026
Same author

Making conservation inclusive and count through globally important agricultural heritage systems as possible other effective area-based conservation measures.

Conservation biology : the journal of the Society for Conservation Biology·2026
Same author

Fluorescence localization and tracking imaging with a spectral-splitting perovskite single-pixel detector.

Nature communications·2026
Same author

A new species of <i>Talaromyces</i> sect. <i>Subinflati</i> discovered in China.

PeerJ·2026
Same author

Buffer-Assisted Epitaxy of Large-Area Ultraflat θ-Tellurene with Mirror Symmetry.

Nano letters·2026
Same author

Ecotypes of triple-negative breast cancer in response to chemotherapy.

Nature·2026

Related Experiment Video

Updated: May 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K

Advances in Functional Nucleic Acid SERS Sensing Strategies.

Lin Shi1,2,3, Yukang Liu1,3, Xiaodong Li1,3

  • 1Center for Biomedical-photonics and Molecular Imaging, Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China.

ACS Sensors
|January 3, 2025
PubMed
Summary
This summary is machine-generated.

Functional nucleic acids, like DNAzymes and aptamers, enable ultrasensitive detection using surface-enhanced Raman spectroscopy (SERS). This review explores their design and applications in diagnostics and environmental monitoring.

Keywords:
CRISPRDNAzymeG-quadruplexaptamerenvironmental monitoringfood safetyfunctional nucleic acidsliquid biopsysensing applicationsurface-enhanced Raman spectroscopy (SERS)

More Related Videos

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.2K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

Related Experiment Videos

Last Updated: May 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.2K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Functional nucleic acids exhibit unique structures beyond conventional DNA amplification.
  • These molecules form complex hybrid structures via Hoogsteen hydrogen bonding.
  • Functional nucleic acid-based surface-enhanced Raman spectroscopy (SERS) offers ultrasensitive and multiplexed detection capabilities.

Purpose of the Study:

  • To review the principles and applications of functional nucleic acids in SERS detection.
  • To elucidate design strategies for functional nucleic acid-based SERS sensors.
  • To highlight advancements in disease diagnosis, environmental monitoring, and food safety.

Main Methods:

  • Overview of structural attributes and SERS sensing mechanisms of functional nucleic acids (DNAzymes, G-quadruplexes, aptamers, CRISPR, origami).
  • Detailed illustration of innovative strategies for constructing functional nucleic acid-based SERS sensors.
  • Comprehensive summary of sensor applications focusing on sensitivity, specificity, and versatility.

Main Results:

  • Functional nucleic acids enable highly sensitive and specific analyte detection through SERS.
  • Various functional nucleic acids demonstrate diverse sensing mechanisms and applications.
  • Innovative sensor designs enhance detection capabilities for critical fields.

Conclusions:

  • Functional nucleic acid-based SERS is a powerful platform for ultrasensitive detection.
  • Significant progress has been made in disease diagnosis, environmental monitoring, and food safety.
  • Future research should address existing challenges and explore new avenues for enhanced analytical performance.