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Updated: May 7, 2025

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Published on: August 13, 2016
Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle
Pingping Cai1, Christian J Casas2, Gabriel Quintero Plancarte2
1Cardiovascular Research Institute, University of California, San Francisco, CA, USA.
Abstract:
Little is known about how distance between homologous chromosomes are controlled during the cell cycle. Here, we show that the distribution of centromere components display two discrete clusters placed to either side of the centrosome and apical/basal axis from prophase to G1 interphase. 4-Dimensional live cell imaging analysis of centromere and centrosome tracking reveals that centromeres oscillate largely within one cluster, but do not cross over to the other cluster. We propose a model of an axis-dependent ipsilateral restriction of chromosome oscillations throughout mitosis.
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