β-cyclodextrin polymers as a new sorbent for solid-phase extraction of xenobiotics in Urine

This study systematically assessed the performance of a newly developed solid-phase extraction (SPE) material, cellulose-supported aminated β-cyclodextrin polymer (amine-β-CDP@Cellulose), in determining 44 xenobiotics, encompassing endocrine-disrupting chemicals (EDCs), pharmaceuticals, and food additives in urine samples. The primary objective of the research was to synthesize a new sorbent, optimize the extraction protocol, and elucidate the underlying adsorption and desorption mechanisms. Following optimization, it was observed that amine-β-CDP@Cellulose achieved recoveries ranging from 80 % to 120 % for 28 of the 44 targeted xenobiotics, with only three compounds showing recoveries below 50 %. The superior extraction performance of this novel material can be attributed to the synergistic effects of its structural components: charged functional groups introduced via the cross-linking agent, the hydrophobic cyclodextrin cavity that facilitates inclusion complexation, and abundant hydroxyl groups that enhance adsorption. Additionally, the study included a comparative analysis between amine-β-CDP@Cellulose and commercially available HLB resins. This comparative analysis revealed that the amine-β-CDP@Cellulose method effectively mitigated matrix interferences while maintaining comparable extraction efficiency to the HLB-based method. Collectively, these findings suggest that amine-β-CDP@Cellulose could serve as a sustainable and cost-effective material for extracting xenobiotics from complex matrices.