Dual-modality flow phantom for ultrasound and optical flow measurements

As ultrasound-compatible flow phantoms are devised for performance testing and calibration, there is a practical need to obtain independent flow measurements for validation using a gold-standard technique such as particle image velocimetry (PIV). In this paper, we present the design of a new dual-modality flow phantom that allows ultrasound and PIV measurements to be simultaneously performed. Our phantom's tissue mimicking material is based on a novel hydrogel formula that uses propylene glycol to lower the freezing temperature of an ultrasound-compatible poly(vinyl) alcohol cryogel and, in turn, maintain the solution's optical transparency after thermocycling. The hydrogel's optical attenuation {1.56 dB cm^-1with 95% confidence interval (CI) of [1.512 1.608]}, refractive index {1.337, CI: [1.340 1.333]}, acoustic attenuation {0.038 dB/(cm × MHz), CI: [0.0368 0.0403]; frequency dependent factor of 1.321, CI: [1.296 1.346]}, and speed of sound {1523.6 m s^-1, CI: [1523.8 1523.4]} were found to be suitable for PIV and ultrasound flow measurements. As an application demonstration, a bimodal flow phantom with spiral lumen was fabricated and used in simultaneous flow measurements with PIV and ultrasound color flow imaging (CFI). Velocity fields and profiles were compared between the two modalities under a constant flow rate (2.5 ml s^-1). CFI was found to overestimate flow speed compared to the PIV measurements, with a 14%, 10%, and 6% difference between PIV and ultrasound for the 60°, 45°, and 30° angles measured. These results demonstrate the new phantom's feasibility in enabling performance validation of ultrasound flow mapping tools.