Inoculation with effective microorganisms agent enhanced fungal diversity in the secondary fermentation process

Microbial inoculations have emerged as a key approach to address the low natural microbial activity of traditional composting technologies. It is crucial for successfully promoting manure composting to understand the influences of microbial inoculations on fungal communities and its mechanisms. To investigate the effects of microbial inoculation on diversity characteristics, tropic mode, and co-occurrence network of fungal communities during composting, an aerobic composting experiment of chicken manure inoculated with microbial agents was performed. The results showed that microbial inoculations enhanced fungal richness and diversity during the secondary fermentation, promoted beneficial fungi, and restrained pathogenic microbes. Microbial inoculation facilitated saprophytic fungi and symbiotic fungi, augmented fungal network complexity and cooperation during the first fermentation, concurrently impeding fungal network complexity and cooperation during the secondary fermentation. These results provide technical guidance for composting process optimization and compost product quality improving, which was beneficial to promote soil quality and mitigating agricultural non-point source pollution.