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Updated: Jun 4, 2025

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
Published on: April 3, 2012
Generation of an induced pluripotent stem cell line from a Kennedy Disease patient with AR mutation
Meng Zhang1, Rui Liu1, Pengpeng Sun1
1Emergency and Critical Care Department, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao 266042, China.
Abstract:
A human induced pluripotent stem cell (iPSC) line was generated from patient with Kennedy Disease (KD), who carried the CAG repeat expansion mutation in AR gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is capable of differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for studing KD in humans.
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