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Related Concept Videos

Spongy Bone01:09

Spongy Bone

All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
The Bone Matrix01:18

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...

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Bone dynamics in zebrafish on low- and high-frequency diets.

Kathryn Bisaha1, Simon Bagatto1, Rebekah Harden1

  • 1University of Akron, Department of Biology, Akron, OH, USA.

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|January 5, 2025
PubMed
Summary

Frequent feeding in zebrafish (Danio rerio) increased weight and length but decreased vertebral bone density and volume. This suggests calorie restriction may negatively impact bone health in zebrafish.

Keywords:
BoneDanio rerioOsteoblastOsteoclastScalemicroCT

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Area of Science:

  • Comparative physiology
  • Zebrafish (Danio rerio) as a model organism
  • Bone biology

Background:

  • Dietary intake significantly influences physiological parameters.
  • Understanding the impact of feeding frequency on bone metabolism is crucial.
  • Zebrafish are a valuable model for studying bone health due to skeletal homology with mammals.

Purpose of the Study:

  • To investigate the effects of different feeding frequencies on bone parameters in zebrafish.
  • To explore the relationship between diet, body composition, and bone density.
  • To hypothesize the role of leptin in diet-induced bone changes.

Main Methods:

  • Zebrafish were divided into two groups: High-frequency (3 meals/day) and Low-frequency (2 meals/week) diets.
  • Fish were fed for 40 days, and total weight and length were measured.
  • Vertebral bone density and volume were assessed.
  • Scale analysis was performed to quantify resorption lacunae.

Main Results:

  • The High-frequency group exhibited significantly greater total weight and length compared to the Low-frequency group (p < 0.01).
  • Vertebral bone density was significantly higher in the High-frequency group (p = 0.0009), while bone volume was significantly lower (p = 0.04).
  • Scales from the Low-frequency group showed a higher density of resorption lacunae (p = 0.02).

Conclusions:

  • Higher feeding frequency in zebrafish leads to increased body size but altered bone characteristics, including higher density and lower volume.
  • Increased bone resorption markers in scales of low-frequency fed fish suggest a catabolic bone state.
  • The findings support a hypothesis that reduced calorie intake, potentially via higher leptin levels, is catabolic to zebrafish bone.