GmRPN11d positively regulates plant salinity tolerance by improving protein stability through SUMOylation

The 26S proteasome is a crucial protease complex responsible for degrading specific proteins to maintain cellular function during salt stress. Previous studies have shown that GmRPN11d, a subunit of the regulatory particle in soybean, is upregulated in response to short-term salt stress. This research discovered that GmRPN11d is localized in the nucleus and cytoplasm, with its expression increasing under high salinity and other stress conditions. Overexpressing GmRPN11d in Arabidopsis and soybean hairy roots significantly improves salt stress tolerance. Examination of physiological indices and expression patterns of salt-responsive marker genes reveals that overexpression of GmRPN11d enhances the ability to scavenge reactive oxygen species, regulates ion balance, exhibits hypersensitivity to ABA, and activates the ABA signaling pathway under salt stress. Additionally, GmRPN11d was demonstrated to interact with the SUMO E3 ligase GmMMS21 in both in vivo and in vitro experiments. This interaction serves to facilitate the SUMOylation of GmRPN11d, ultimately contributing to its stability when faced with salt stress. Taken together, these findings highlight the role of GmRPN11d in promoting plant salt tolerance through SUMOylation, mediated by GmMMS21. This study provides valuable insights into modifying the 26S proteasome subunit in soybean, offering a potential target gene for developing genetically modified salt-resistant crops.