LCMS Detection and Characterization of In Vitro Metabolites of Isotonitazene, a Targeted Strategy for Novel...

Area of Science:

• Pharmacology
• Veterinary Science
• Analytical Chemistry

Background:

• Novel psychoactive substances, including benzimidazoles, are increasingly misused.
• Concerns exist regarding doping in animal sports like camel racing.
• Understanding substance metabolism is key for detection and animal welfare.

Purpose of the Study:

• To identify in vitro metabolites of isotonitazene in camels.
• To establish metabolic pathways for isotonitazene in this species.
• To support the development of doping control strategies in camel racing.

Main Methods:

• In vitro studies using homogenized camel liver.
• Metabolite extraction and analysis via high-resolution accurate mass LC-MS (Thermo Fisher Orbitrap Exploris).
• Data processing using Compound Discoverer software.

Main Results:

• Nine isotonitazene metabolites were identified in vitro.
• Primary phase I metabolic pathways include dealkylation and hydroxylation.
• Identified metabolites are suitable for long-term detection of isotonitazene use.

Conclusions:

• Advanced LC-MS techniques successfully identified isotonitazene metabolites in camels.
• Isotonitazene abuse potential necessitates robust doping control.
• Findings are critical for regulating camel racing and enhancing animal welfare.