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Comprehensive, Quantitative Analysis of SRM 1950: the NIST Human Plasma Reference Material
Rupasri Mandal1, Jiamin Zheng1, Lun Zhang1
1Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.
Analytical Chemistry
|January 6, 2025
Summary
This study comprehensively analyzed NIST human plasma SRM 1950, quantifying 1058 metabolites using multiple analytical techniques. The results provide valuable benchmarks for metabolomics calibration and comparison efforts.
Area of Science:
- Metabolomics
- Analytical Chemistry
- Biochemistry
Background:
- Targeted metabolomics relies on accurate analytical methods for metabolite quantification.
- Comprehensive metabolome coverage is often achieved by combining multiple analytical techniques.
- The NIST human plasma reference material, SRM 1950, is widely used for calibration and validation.
Purpose of the Study:
- To comprehensively and quantitatively characterize NIST human plasma SRM 1950.
- To provide a large, well-validated list of metabolite concentration values (benchmarks).
- To aid the metabolomics community in calibration and comparison efforts.
Main Methods:
- Combined four analytical platforms: high-resolution NMR spectroscopy, direct injection tandem MS (DI-MS/MS), LC-MS/MS, and ICP-MS.
- Utilized eight validated analytical assays for accurate quantitative measurements.
- Performed computer-aided literature mining to identify previously quantified metabolites.
Main Results:
- Quantitatively characterized 728 unique metabolites using the combined analytical platforms and assays.
- Identified an additional 330 unique metabolites through literature mining.
- Generated a high-confidence list of 1058 metabolites/metabolite species in SRM 1950, including diverse chemical classes.
Conclusions:
- This study presents the most complete quantitative characterization of SRM 1950 to date.
- The generated data provides essential benchmarks for the metabolomics community.
- An online database (SRM1950-DB) is freely available with detailed metabolite information.
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