Related Experiment Video

Updated: Jun 3, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

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The [2Fe-2S] cluster of mitochondrial outer membrane protein mitoNEET has an O2-regulated nitric oxide access tunnel

Thao Nghi Hoang1,2, Meritxell Wu-Lu3, Alberto Collauto4

  • 1Institute of Pharmaceutical Science, King's College London, UK.

FEBS Letters
|January 6, 2025
PubMed

Insights

Researchers discovered a nitric oxide (NO) access site on the mitoNEET protein

Area of Science:

  • Mitochondrial biology
  • Biochemistry
  • Cell signaling

Background:

  • The iron-sulphur protein mitoNEET is involved in mitochondrial respiration and targeted by pioglitazone.
  • Its precise molecular function remains unknown despite extensive research.

Purpose of the Study:

  • To elucidate the molecular function of mitoNEET.
  • To investigate the interaction of mitoNEET with nitric oxide (NO).

Main Methods:

  • Interdisciplinary approach combining biophysical and biochemical techniques.
  • Investigated the effect of oxygen and pioglitazone on NO binding to the mitoNEET [2Fe-2S] cluster.

Main Results:

  • Identified a specific site for nitric oxide (NO) access to the mitoNEET [2Fe-2S] cluster.
  • Oxygen (O2) and pioglitazone were found to block NO access to the cluster.
  • This suggests a role for mitoNEET in sensing hypoxia and NO signaling.

Conclusions:

  • MitoNEET's [2Fe-2S] cluster is involved in mitochondrial signal transduction.
  • Mitochondria may sense hypoxia via O2-mediated protection of the mitoNEET cluster.
  • This highlights a new role for [Fe-S] clusters in gasotransmitter signaling.
Keywords:
H2Sgasotransmitterhypoxiairon–sulphur clustermitoNEETnitric oxide

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