Exploring the potential of hydrolytic enzymes combined with antibacterial agents to disrupt pathogenic biofilms and...

Biofilms are bacterial communities encapsulated in a self-produced extracellular polymeric matrix comprising carbohydrates, proteins, lipids, and DNA. This matrix provides structural integrity while significantly enhancing bacterial antibiotic resistance, presenting substantial disinfection challenges. The persistence of biofilm-associated infections and foodborne outbreaks underscores the need for more effective disinfection strategies. Conventional antibacterial agents often are less effective against biofilm-protected cells compared to their efficacy against planktonic (non-attached) bacteria. Integrating hydrolytic enzymes, such as cellulases, proteases, and DNases, into disinfection protocols offers a promising approach by breaking down the biofilm matrix to expose the bacteria. However, the follow-up use of antibacterial agents is important, as enzymes alone do not possess bactericidal properties. Unlike traditional disinfectants, natural antibacterial agents work synergistically with enzymes, enhancing biofilm disruption without compromising the enzymatic activity through oxidation. This review offers a comprehensive analysis of the current knowledge and potential of combining hydrolytic enzymes with disinfectants to disrupt biofilms and eradicate the released bacterial cells, emphasizing applications for clinical and foodborne pathogens.