Recent Advances in Biosensors Based on Hybridization Chain Reaction and Silver Nanoclusters

Area of Science:

• Biotechnology
• Nanotechnology
• Analytical Chemistry

Background:

• Hybridization chain reaction (HCR) and DNA-templated silver nanoclusters (AgNCs) are advanced biosensing tools.
• HCR provides cascade amplification, while AgNCs offer unique signal transduction (fluorogenic, electrochemical).
• Integrating HCR and AgNCs into hybrid biosensors promises enhanced detection capabilities.

Purpose of the Study:

• To provide a comprehensive review of HCR-AgNCs biosensors.
• To address the lack of systematic guidelines for HCR-AgNCs system development.
• To facilitate innovation and guide future research in hybrid biosensing.

Main Methods:

• Review of existing literature on HCR-AgNCs biosensors.
• Analysis of working principles, performance factors, and complementary features.
• Assessment of distinct sensing mechanisms, applications, and challenges.

Main Results:

• HCR-AgNCs biosensors demonstrate significant potential for enhanced sensing.
• Various sensing mechanisms (fluorogenic, electrochemical) and applications are identified.
• Challenges in adopting hybrid sensors and insights for development are discussed.

Conclusions:

• The synergistic combination of HCR and AgNCs offers a promising platform for next-generation biosensing.
• Guidelines and future research directions, including multiplex assays and emerging technologies, are proposed.
• HCR-AgNCs biosensors hold potential for critical applications in healthcare and environmental monitoring.