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Circle Padlock-Mediated Catalytic Hairpin Assembly Cooperating Primer Exchange Reaction for Sensitive and Label-Free
Meiyan Chen1, Qiongdan Kang1, Annan Zhang1
1Department of Gastroenterology, Xiamen University Affiliated Chenggong Hospital, Xiamen City, Fujian Province 361003, China.
ACS Omega
|January 6, 2025
Summary
This study presents a novel fluorescent assay for highly sensitive microRNA detection. The method shows promise for early gastric ulcer diagnosis using serum samples within hours.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- MicroRNAs (miRNAs) are crucial regulators of gene expression and cell function.
- miRNAs are recognized as potential biomarkers for diseases like gastric ulcers.
- Sensitive and specific miRNA detection is vital for clinical diagnosis and therapy.
Purpose of the Study:
- To develop a label-free, low-background fluorescent assay for sensitive miRNA detection.
- To enable early diagnosis of diseases such as gastric ulcers.
Main Methods:
- Coupling target miRNA-triggered padlock probe cyclization with catalytic hairpin assembly (CHA).
- Utilizing primer exchange reaction (PER) for signal amplification.
- G-quadruplex sequence generation and thioflavin T binding for fluorescence signal.
Main Results:
- Achieved highly sensitive detection of target miRNAs.
- Demonstrated a low-background fluorescent signal.
- Successfully detected miRNAs in human serum samples within hours.
Conclusions:
- The developed assay offers a sensitive and rapid method for miRNA detection.
- This technique holds significant potential for the early clinical diagnosis of diseases like gastric ulcers.
