Advanced reactivity-based sequencing methods for mRNA epitranscriptome profiling

Area of Science:

• Molecular Biology
• Genetics
• Biochemistry

Background:

• Over 170 known RNA modifications contribute to gene expression regulation, forming the epitranscriptome.
• Understanding these dynamic and reversible modifications is crucial for deciphering gene expression control.
• Advances in detection methods have spurred research into the roles of RNA modifications.

Purpose of the Study:

• To review recent advancements in sequencing methods for RNA modification detection.
• To highlight enzyme- and chemical-assisted techniques for characterizing RNA modifications.
• To deepen the understanding of the biological functions and roles of RNA modifications.

Main Methods:

• Review of enzyme-assisted sequencing strategies for RNA modification analysis.
• Survey of chemical-assisted sequencing approaches for RNA modification detection.
• Focus on reactivity-based techniques for detailed RNA modification characterization.

Main Results:

• Significant progress in enzyme- and chemical-assisted sequencing methods has been achieved.
• These reactivity-based techniques offer new opportunities for detailed RNA modification analysis.
• The review consolidates current knowledge on the biological significance of RNA modifications.

Conclusions:

• Enzyme- and chemical-assisted sequencing are powerful tools for studying the epitranscriptome.
• Further research using these methods will enhance our understanding of RNA modification functions.
• The epitranscriptome plays a vital role in regulating gene expression and biological processes.