Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

4.7K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immune Mechanisms of Heart Valve Development, Homeostasis, and Disease.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

A Decade of Leadership and Impact: Celebrating 10 Years of the ORS Spine Section.

JOR spine·2026
Same author

Advancing Basic and Preclinical Spine Research: Highlights From the ORS PSRS 7th International Spine Research Symposium.

JOR spine·2026
Same author

Lipopolysaccharides increase the resorption levels and affect zebrafish scales de novo bone formation.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2025
Same author

Immunomodulatory inhibition of osteoclastogenesis by a marine microalgal ethanol fraction targeting T-cells, antigen presentation, and macrophage fate.

Frontiers in immunology·2025
Same author

Injury Induces More Severe Biomechanical Changes in Middle-Aged and Geriatric Lumbar Spines in a Mouse Ex Vivo Model.

JOR spine·2025
Same journal

Utilizing Single-Cell and Transcriptomic Data to Identify Mitochondrial Pathway-Associated Prognostic Genes and Their Regulatory Mechanisms of Action in Ovarian Cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Gene-Environment Interactions in Childhood Asthma: From Prenatal Exposures to Targeted Interventions.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

m6A-Modified KLF11 Inhibits Macrophage Foam Cell Formation by Transcriptionally Suppressing ATP5B Expression in Atherosclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Dissecting Allele-Specific Expression (ASE) in Landrace × Meishan Cross Pig Fetal-Placental Tissues: Distribution, Functional Split, and Mechanisms.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Blockade of FGFR1 Trafficking to the Cell Surface Results in the Partial Mistargeting of the Receptor to Peroxisomes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

A Myocyte-Enriched Long Non-Coding RNA NRMLncR Enhances Myogenesis in Mouse.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury
07:25

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury

Published on: May 25, 2017

11.5K

Klotho mutation does not accelerate intervertebral disc aging in mice.

Justin Hong1, Veeraj Shah1, Ravi Krishnan1

  • 1HSS Research Institute, Hospital for Special Surgery, New York, New York, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|January 6, 2025
PubMed
Summary
This summary is machine-generated.

Klotho deficiency does not accelerate disc degeneration in mice. Klotho (Kl) gene deficiency did not impact lumbar disc structure or molecular markers in young mice, suggesting disc cells do not express Klotho.

Keywords:
accelerated agingannulus fibrosusdegenerationintervertebral discklothonucleus pulposussonic hedgehog

More Related Videos

A Mouse Model of Lumbar Spine Instability
05:28

A Mouse Model of Lumbar Spine Instability

Published on: April 23, 2021

7.7K
Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

2.1K

Related Experiment Videos

Last Updated: May 3, 2026

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury
07:25

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury

Published on: May 25, 2017

11.5K
A Mouse Model of Lumbar Spine Instability
05:28

A Mouse Model of Lumbar Spine Instability

Published on: April 23, 2021

7.7K
Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

2.1K

Area of Science:

  • Biogerontology
  • Musculoskeletal Biology
  • Molecular Biology

Background:

  • Aging is a primary risk factor for intervertebral disc degeneration (IDD) and associated back pain.
  • Key features of disc pathology include cellular loss, structural disorganization, and reduced disc height.
  • Understanding the molecular mechanisms of IDD is crucial for developing effective treatments.

Purpose of the Study:

  • To evaluate the suitability of Klotho-deficient (KlKl/KlKl) mice for studying accelerated disc degeneration.
  • To investigate whether Klotho deficiency impacts the structural, morphological, or molecular characteristics of intervertebral discs.

Main Methods:

  • Characterization of lumbar discs from eight-week-old KlKl/KlKl mice and wild-type controls.
  • Assessment of structural, morphological, and molecular markers of disc health.
  • Multiplex quantitative PCR (qPCR) to detect Klotho isoform expression in disc cells.

Main Results:

  • No significant structural, morphological, or molecular differences were observed in the lumbar discs of KlKl/KlKl mice compared to controls.
  • Multiplex qPCR analysis revealed no detectable Klotho isoforms in the intervertebral disc cells.
  • These findings indicate that Klotho deficiency does not induce accelerated disc pathologies in this model.

Conclusions:

  • Klotho-deficient mice do not exhibit accelerated intervertebral disc degeneration.
  • The absence of Klotho expression or response in disc cells explains the lack of a disc phenotype in Klotho-deficient mutants.
  • These findings suggest that Klotho may not play a direct role in the aging of intervertebral discs.