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Updated: May 7, 2025

Optimized Intravenous Injection in Adult Zebrafish

Optimized Intravenous Injection in Adult Zebrafish

Published on: December 20, 2024

Optimized Intravenous Injection in Adult Zebrafish.

Fangfang He¹, Lingge Tu¹, Leslie Chan²

¹Department of Medicine, School of Clinical Medicine, LKS Faculty of Medicine, The University of Hong Kong.

Journal of Visualized Experiments : Jove

|January 6, 2025

Summary

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This summary is machine-generated.

We optimized intravenous (IV) injection for adult zebrafish, improving survival and engraftment efficiency for drug delivery and stem cell research. This refined IV technique enhances zebrafish models for broader research applications.

Area of Science:

Zebrafish models
Transplantation biology
Drug delivery systems

Background:

Intravenous (IV) injection is standard for systemic delivery in mammals.
IV injection in adult zebrafish is challenging due to small size and vasculature.
Existing methods like intracardiac and retro-orbital injections have limitations.

Purpose of the Study:

To develop and optimize an intravenous injection technique for adult zebrafish.
To overcome anatomical challenges for effective substance delivery.
To improve survival rates and engraftment efficiency compared to other methods.

Main Methods:

Developed a refined and optimized IV injection procedure for adult zebrafish.
Administered whole kidney marrow cells and FITC-dextran dye via IV injection.

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Last Updated: May 7, 2025

Optimized Intravenous Injection in Adult Zebrafish

Optimized Intravenous Injection in Adult Zebrafish

Published on: December 20, 2024

Intraperitoneal Injection into Adult Zebrafish

Intraperitoneal Injection into Adult Zebrafish

Published on: August 30, 2010

Author Spotlight: Novel Intraperitoneal Injection Method for Zebrafish Cardiotoxicity Models

Author Spotlight: Novel Intraperitoneal Injection Method for Zebrafish Cardiotoxicity Models

Published on: March 29, 2024

Utilized fluorescence microscopy to track injected substances and assess engraftment.

Main Results:

Successfully delivered cells and dye into adult Casper zebrafish via IV injection.
Demonstrated successful engraftment and systemic distribution of injected materials.
Achieved improved survival rates and engraftment efficiency compared to intracardiac and retro-orbital injections.

Conclusions:

The optimized IV injection technique is effective for adult zebrafish.
This method enhances precision, survival, and engraftment efficiency.
Holds potential for large-scale drug screening and studying biological processes in zebrafish.