Energy-Transfer-Based Dual-Mode PEC-ECL Biosensor for Acetamiprid Analysis Sensitized by Two-Step DNA Circuit...

Area of Science:

• Analytical Chemistry
• Materials Science
• Biotechnology

Background:

• Accurate determination of acetamiprid is crucial for ensuring food safety.
• Existing detection methods may lack the required sensitivity or specificity.
• Development of advanced biosensors is needed for effective pesticide monitoring.

Purpose of the Study:

• To develop an energy-transfer-based dual-mode biosensor for sensitive acetamiprid detection.
• To integrate aptamer recognition with DNA circuit amplification for enhanced signal generation.
• To utilize zinc-based metal-organic frameworks (Zn-MOFs) and Pt@Cu2O nanocrystals for dual photoelectrochemical (PEC) and electrochemiluminescent (ECL) sensing.

Main Methods:

• Fabrication of a dual-mode biosensor using Zn-MOFs as donors and Pt@Cu2O nanocrystals as acceptors.
• Incorporation of aptamer recognition and a two-step DNA circuit amplification strategy (entropy-driven DNA cycle and DNA walker).
• Utilizing nicking endonuclease to generate single-stranded DNA on Zn-MOFs and competitive hybridization for signal transduction.

Main Results:

• The biosensor demonstrated sensitive detection of acetamiprid via both PEC and ECL modes.
• Achieved ultra-low detection limits of 20.2 fM (PEC) and 17.5 fM (ECL) within a wide linear range (0 to 1 × 10^-9 M).
• The developed system exhibited excellent specificity, reproducibility, and practicality for real-world applications.

Conclusions:

• The energy-transfer-based dual-mode biosensor effectively quantifies acetamiprid with high sensitivity and accuracy.
• The combination of advanced materials, DNA amplification, and dual-mode sensing shows significant potential for pesticide monitoring in food safety.
• This biosensor offers a promising tool for safeguarding public health through reliable food analysis.