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Updated: Jun 3, 2025
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Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
Published on: August 8, 2022
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Systematic development and bioprinting of novel nanostructured multi-material bioinks for bone tissue engineering
Jannika T Korkeamäki1, Ahmad Rashad1, Miina Ojansivu1
1Center of Translational Oral Research (TOR), Department of Clinical Dentistry, University of Bergen, Bergen, Norway.
Biofabrication
|January 6, 2025
Summary
This study developed advanced alginate-gelatin bioinks for bone tissue engineering. Optimized bioinks with cellulose nanofibrils and nano-hydroxyapatite improved printability and cell viability, showing osteogenic potential in human bone marrow stromal cells.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Developing functional bioinks is crucial for bone tissue engineering.
- Alginate-gelatin (Alg-Gel) bioinks require systematic optimization for cell response.
- Understanding long-term cell behavior within bioinks is essential.
Purpose of the Study:
- To systematically develop and characterize alginate-gelatin-based bioinks for bone tissue engineering.
- To assess human bone marrow stromal cell (hBMSC) responses to bioinks during short- and long-term culture.
- To investigate the osteogenic potential and released cell functionality of optimized bioinks.
Main Methods:
- Iterative development of bioink formulations (Generations 1-3) incorporating cellulose nanofibrils (CNFs), nano-hydroxyapatite (nHA), and RGD-functionalized alginate.
- Evaluation of bioink properties including viscosity, printability, and structural integrity.
- Assessment of hBMSC viability, proliferation, osteogenic differentiation (protein and gene expression), and functionality after release.
Main Results:
- Incorporating CNFs improved viscosity and printability without compromising hBMSC viability.
- Addition of nHA further enhanced viscosity and printability; alginate concentration adjustment improved long-term structural stability.
- The final RGD-Alg-Gel-CNF-nHA bioink demonstrated excellent printability, structural integrity, high cell viability, and supported osteogenic differentiation of hBMSCs.
- Released cells exhibited distinct osteogenic differentiation patterns compared to cells cultured within the 3D bioink structure.
Conclusions:
- A systematic, multi-generational approach successfully optimized Alg-Gel bioinks for bone tissue engineering.
- The developed RGD-Alg-Gel-CNF-nHA bioink shows significant potential for bone regeneration applications.
- The bioprinting process and bioink composition influence the osteogenic differentiation of encapsulated and released cells.
