Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

pH Homeostasis01:31

pH Homeostasis

Acid-base homeostasis is essential for maintaining normal physiological activities in humans. The pH of various body fluids is strictly regulated because it is critical for the optimal activity of enzymes involved in metabolic reactions. Enzymes are basically proteins, so, any significant change in pH can affect their structure and activity. In humans, pH is regulated using three primary mechanisms— chemical buffer systems, respiratory regulation, and renal regulation.
Respiratory Regulation of...
Acids, Bases and Neutralization Reactions01:27

Acids, Bases and Neutralization Reactions

Acids and bases play several important roles in biology. The pH of a biological system can significantly impact the function of biological molecules, including enzymes, proteins, and nucleic acids. For example, enzymes have optimal pH ranges for their activity, and changes in pH can denature or alter their structure, affecting their function. Acids and bases also play a crucial role in cellular signaling and communication. The pH of the extracellular fluid around cells can influence the...
Enhanced Elimination of Poison01:26

Enhanced Elimination of Poison

Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures.
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...
Compensation Mechanisms01:28

Compensation Mechanisms

The human body employs intricate mechanisms to counteract changes in blood pH, preventing conditions like acidosis (pH < 7.35) and alkalosis (pH > 7.45). These compensatory responses aim to restore normal arterial blood pH by engaging respiratory or renal systems, depending on the source of the imbalance.
Respiratory Compensation
This mechanism addresses metabolic-induced pH imbalances by adjusting breathing rates. Respiratory compensation begins within minutes of detecting a pH...
Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arabidopsis inositol polyphosphate kinase activities regulate COP9 deneddylation functions in phosphate homeostasis.

Journal of integrative plant biology·2026
Same author

Pooled safety analysis of lurbinectedin plus irinotecan in patients with advanced solid tumors.

Investigational new drugs·2026
Same author

Metabolic Kidney Disease: A New Concept in the Interaction Between Obesity, Prediabetes, Diabetes and Liver Dysfunction.

Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia·2026
Same author

Acute hemodynamic effects of a novel algorithm for cardiac resynchronization therapy optimization: Results from the BIO|Adapt study.

Heart rhythm O2·2026
Same author

Care Models for the Genetic Evaluation of Dilated Cardiomyopathy at Sites of the DCM Consortium.

medRxiv : the preprint server for health sciences·2026
Same author

Nuclear speckle dynamics are controlled by polyphosphate inhibition of CLK proteins.

Nucleic acids research·2026

Related Experiment Video

Updated: Jun 7, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
08:58

High-Throughput Measurement and Classification of Organic P in Environmental Samples

Published on: June 8, 2011

12.9K

Optimized biochemical method for human Polyphosphate quantification.

Blanca Lázaro1, Ana Sarrias1, Francisco J Tadeo1

  • 1Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universitat Internacional de Catalunya. 08195 Barcelona, Spain.

Methods (San Diego, Calif.)
|January 6, 2025
PubMed
Summary

Researchers developed a new method to extract and quantify polyphosphate (polyP) from human cells, revealing its structure and variations. This breakthrough offers a standardized tool for studying polyP

Keywords:
Gold-standardHuman-cellsNMRPolyphosphate

More Related Videos

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

8.5K
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
06:49

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Published on: May 8, 2020

4.6K

Related Experiment Videos

Last Updated: Jun 7, 2026

High-Throughput Measurement and Classification of Organic P in Environmental Samples
08:58

High-Throughput Measurement and Classification of Organic P in Environmental Samples

Published on: June 8, 2011

12.9K
Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

8.5K
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
06:49

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

Published on: May 8, 2020

4.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Polyphosphate (polyP) is a biopolymer with diverse biological roles and involvement in diseases.
  • Understanding polyP's structure and function in human cells is limited by inadequate extraction and quantification methods.

Purpose of the Study:

  • To develop and validate a highly efficient method for extracting, purifying, and quantifying polyphosphate from human cell lines.
  • To characterize the structure of mammalian polyP.
  • To investigate variations in intracellular polyP levels and their correlation with enzyme expression in human cells.

Main Methods:

  • Optimization of polyP extraction, purification, and quantification from human cell lines.
  • Utilizing Nuclear Magnetic Resonance (NMR) for structural analysis.
  • Application of the optimized method to common human cell lines.

Main Results:

  • The developed method achieved 3 to 100 times higher polyP extraction yields compared to previous methods.
  • Nuclear Magnetic Resonance (NMR) confirmed mammalian polyP is predominantly a linear, unbranched polymer.
  • Significant variations in intracellular polyP levels were observed across different human cell lines, correlating with polyP-converting enzyme expression.

Conclusions:

  • The optimized method provides a standardized and efficient tool for polyP research.
  • Mammalian polyP is primarily linear, and its intracellular levels vary significantly and are linked to enzyme activity.
  • Further exploration of polyP's role in human physiology and pathology is facilitated by this new methodology.