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Two FAM134B isoforms differentially regulate ER dynamics during myogenesis.

Viviana Buonomo1,2, Kateryna Lohachova3,4, Alessio Reggio1,5

  • 1Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, Italy.

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|January 6, 2025
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Summary
This summary is machine-generated.

The study reveals how FAM134B isoforms regulate endoplasmic reticulum (ER) remodeling during muscle cell differentiation. A shift towards FAM134B2 drives ER restructuring via ER-phagy, maintaining muscle cell homeostasis.

Keywords:
AutophagyEndoplasmic ReticulumFAM134BMyogenesisReticulophagy

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Muscle Physiology

Background:

  • Endoplasmic reticulum (ER) plasticity and ER-phagy are crucial for cellular homeostasis.
  • FAM134B is an ER-phagy receptor involved in ER remodeling.

Purpose of the Study:

  • Investigate the role of FAM134B isoforms in ER remodeling during myogenesis.
  • Elucidate the interplay between FAM134B isoforms and ER-phagy in differentiating myoblasts.

Main Methods:

  • Studied FAM134B isoform dynamics during myogenesis.
  • Utilized knockout and re-expression models in myotubes.
  • Analyzed ER morphology and proteome landscape.

Main Results:

  • Canonical FAM134B1 is degraded while FAM134B2 is upregulated during myogenesis.
  • FAM134B2 promotes ER reshaping and restructuring via ER-phagy.
  • FAM134B2 re-expression rescues aberrant proteome and ER dilation in FAM134B knockout myotubes.

Conclusions:

  • Fine-tuning of FAM134B isoforms orchestrates ER dynamics during myogenesis.
  • FAM134B2 plays a key role in ER homeostasis in muscle cells.
  • Understanding these mechanisms provides insights into muscle cell biology.