Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tumor Immunotherapy01:27

Tumor Immunotherapy

472
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
472
Cancer Vaccines01:30

Cancer Vaccines

337
Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
337
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual salt bridges govern proton gating and calcium leak in <i>Bs</i>YetJ across bilayers and live cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

cGAMP-Loaded M2e Nanovaccine Elicits Cross-Reactive Immunity and Mitigates H6N1 Avian Influenza Infection in Chickens.

International journal of nanomedicine·2026
Same author

Matrix Control of Solvent and Electron Flow in a Nonheme Diiron Nitrite Reductase.

JACS Au·2026
Same author

Mesoscopic Inhomogeneities in Ethanol-Water Mixtures: Are They Nanobubbles, Impurity Aggregates, or Nanoscale Gas-Water Composite Structures?

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Single-Carbon Bridged Pentacene Dimers Enable Efficient Singlet Fission and Quintet State Stabilization.

Journal of the American Chemical Society·2026
Same author

Targeted computational design of an interleukin-7 superkine with enhanced folding efficiency and immunotherapeutic efficacy.

eLife·2026

Related Experiment Video

Updated: Jun 3, 2025

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.5K

Endotoxin-Free Outer Membrane Vesicles for Safe and Modular Anticancer Immunotherapy.

Mei-Yi Chen1,2,3, Ting-Wei Cheng1,2, Yi-Chung Pan1,2

  • 1Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nangang (Nankang) Dist., Taipei City 115201, Taiwan.

ACS Synthetic Biology
|January 7, 2025
PubMed
Summary

Removing lipopolysaccharide (LPS) from bacterial outer membrane vesicles (OMVs) significantly enhances their safety and anticancer efficacy. LPS-free OMVs demonstrate improved tolerability and superior tumor-inhibiting effects compared to traditional OMVs.

Keywords:
bacteria engineeringimmunotherapylipopolysaccharides (LPS)nanoparticlesouter membrane vesicle (OMV)

More Related Videos

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

9.4K
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
08:02

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Published on: September 23, 2021

2.5K

Related Experiment Videos

Last Updated: Jun 3, 2025

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.5K
Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

9.4K
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
08:02

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Published on: September 23, 2021

2.5K

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Immunology

Background:

  • Bacterial outer membrane vesicles (OMVs) are explored as drug delivery systems for cancer therapy.
  • High lipopolysaccharide (LPS) content in OMVs poses safety and regulatory hurdles.
  • Modifying OMVs to reduce LPS is crucial for clinical translation.

Purpose of the Study:

  • To develop and evaluate LPS-attenuated and LPS-free OMVs for cancer treatment.
  • To assess the impact of LPS modification on OMV properties and therapeutic potential.
  • To investigate the safety, efficacy, and mechanisms of LPS-free OMVs.

Main Methods:

  • Production of LPS-attenuated and LPS-free bacterial outer membrane vesicles (OMVs).
  • Systematic assessment of physicochemical characteristics, protein content, and immune stimulation.
  • Evaluation of maximal tolerated dose, anticancer efficacy, and tumor immune cell infiltration.
  • Functionalization of LPS-free OMVs with an IL-2 variant protein (Neo-2/15).

Main Results:

  • LPS removal increased the maximal tolerated dose of OMVs by over 25-fold.
  • LPS-free OMVs showed superior anticancer effects compared to wild-type OMVs at comparable safety levels.
  • LPS removal prevented LPS-induced immune cell death and improved tumor immune cell infiltration.
  • Functionalized LPS-free OMVs enhanced tumor growth inhibition and lymphocyte infiltration.

Conclusions:

  • LPS modification is critical for improving the safety and efficacy of OMVs in cancer therapy.
  • LPS-free OMVs represent a safer and more potent platform for anticancer drug delivery.
  • Functionalized LPS-free OMVs hold significant promise for improved cancer treatment strategies.