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Updated: May 7, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Long noncoding RNA, PURPL is associated with aneuploidy and its magnitude of expression level is dependent on P53

Pushkar Malakar¹

¹Department of Medical Biotechnology, School of Biological Sciences, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), Kolkata, India.

Frontiers in Cell and Developmental Biology

|January 7, 2025

Abstract

Introduction:

Long non-coding RNAs (lncRNAs) are a fascinating, but still largely uncharacterized, class of genes. Recently, lncRNAs have attracted significant attention due to their emerging functions in development and disease. The role of lncRNAs in chromosome instability or aneuploidy is not extensively studied.

Methods:

We started with the objective of characterizing lncRNAs that play an important role in chromosome instability (CIN) or aneuploidy. Here, we report the initial functional characterization of PURPL in the context of chromosomal instability or aneuploidy.

Results:

We report the over-expression of lncRNA PURPL in three experimental models of chromosomal instability, or aneuploidy. In addition, the study also showed that the extent or magnitude of PURPL expression is dependent upon p53 status. Our research also showed that turning off PURPL is enough to create a CIN phenotype in RPE-1 cell lines that were previously karyotypically stable. Moreover, PURPL knockdown cells are more sensitive to CIN or aneuploidy inducers.

Discussion:

These findings show that PURPL is essential for maintaining chromosomal or genomic stability in mammalian cells. Collectively, the study demonstrated that lncRNA-PURPL significantly contributes to CIN, or aneuploidy.

Keywords:

PURPL altered nuclear shape, and aneuploidy chromosomal instability long noncoding RNA multinucleation p53

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

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