Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...
Tissue Transplantation01:24

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment Responses in Histologic Versus Molecular Diagnoses of Lung Rejection.

Transplant international : official journal of the European Society for Organ Transplantation·2024
Same author

X-linked retinoschisis: mutation spectrum and genotype-phenotype relationship in an Italian pediatric cohort.

Ophthalmic genetics·2022
Same author

The Ryder Cup of lung transplantation.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2022
Same author

The effects of surface electrical stimulation plus voice therapy in Parkinson's disease.

The Journal of laryngology and otology·2022
Same author

Once in a blue moon: Primaquine-induced methemoglobinemia - A case report.

Respiratory medicine case reports·2022
Same author

EJ-antisynthetase syndrome presenting as severe acute respiratory distress syndrome.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2021

Related Experiment Video

Updated: Jul 11, 2026

Study of Experimental Organ Donation Models for Lung Transplantation
08:56

Study of Experimental Organ Donation Models for Lung Transplantation

Published on: March 15, 2024

716

Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.

H Beeckmans1, A Pagliazzi2, P Kerckhof1

  • 1Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of CHROMETA, KU Leuven, Leuven, Belgium.

Frontiers in Transplantation
|January 7, 2025
PubMed
Summary

Donor-derived cell-free DNA (dd-cfDNA) shows ongoing lung allograft injury in patients with chronic lung allograft dysfunction (CLAD). While dd-cfDNA levels increased with CLAD severity, high variability limits its current use for early detection.

Keywords:
CLADbiomarkerdd-cfDNAlung transplantationrejection

More Related Videos

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death
09:54

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death

Published on: August 15, 2022

1.7K
Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation
07:28

Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation

Published on: October 11, 2024

274

Related Experiment Videos

Last Updated: Jul 11, 2026

Study of Experimental Organ Donation Models for Lung Transplantation
08:56

Study of Experimental Organ Donation Models for Lung Transplantation

Published on: March 15, 2024

716
Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death
09:54

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death

Published on: August 15, 2022

1.7K
Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation
07:28

Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation

Published on: October 11, 2024

274

Area of Science:

  • Transplantation Immunology
  • Biomarker Discovery
  • Genomics

Background:

  • Chronic lung allograft dysfunction (CLAD) limits long-term survival post-lung transplantation.
  • CLAD presents as bronchiolitis obliterans syndrome (BOS) or restrictive allograft syndrome (RAS).
  • Donor-derived cell-free DNA (dd-cfDNA) may indicate subclinical allograft injury.

Purpose of the Study:

  • To assess plasma dd-cfDNA levels as a biomarker for lung allograft health.
  • To evaluate dd-cfDNA across stable, preclinical, and established CLAD stages.
  • To compare dd-cfDNA in BOS and RAS phenotypes.

Main Methods:

  • Prospective study of 20 lung transplant recipients with CLAD.
  • Collected plasma samples at three time points: stable, preclinical CLAD, and established CLAD.
  • Measured dd-cfDNA levels, excluding concurrent infection or acute rejection.

Main Results:

  • Elevated dd-cfDNA detected in 47% of stable, 66% of preclinical, and 71% of established CLAD samples.
  • dd-cfDNA levels indicated ongoing allograft injury across CLAD spectrum.
  • High intra- and interpatient variability observed; no significant difference between BOS and RAS (p=0.25), though higher range in RAS.

Conclusions:

  • dd-cfDNA can detect ongoing allograft injury in CLAD patients.
  • Further research is needed to improve early CLAD detection using dd-cfDNA.
  • Variability in dd-cfDNA requires further investigation for clinical utility.