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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Immunocytochemistry and Immunohistochemistry01:22

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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

Updated: Jun 3, 2025

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach
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The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging

Ziv Yaniv1, Ifeanyichukwu U Anidi2, Leanne Arakkal3

  • 1Bioinformatics and Computational Bioscience Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Arxiv
|January 7, 2025
PubMed
Summary

The iterative bleaching extends multiplexity (IBEX) Knowledge-Base supports researchers using advanced immunofluorescence imaging. It promotes open science by sharing data, protocols, and fostering community for faster, more confident scientific discovery.

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Area of Science:

  • Life Sciences
  • Biotechnology
  • Microscopy

Background:

  • Iterative bleaching extends multiplexity (IBEX) is a method for advanced 2D and 3D immunofluorescence imaging.
  • Researchers require centralized resources for adopting and sharing complex imaging techniques.

Purpose of the Study:

  • To establish a central Knowledge-Base for IBEX and related immunofluorescence imaging methods.
  • To facilitate open science practices throughout the research lifecycle.
  • To foster a community of practice for researchers.

Main Methods:

  • Modeled after open-source software communities, the Knowledge-Base integrates a development platform (GitHub), a static website, and data archiving services.
  • Provides validation data for reagents, including primary and secondary antibodies.
  • Offers a platform for sharing protocols, videos, datasets, software, and publications.

Main Results:

  • The Knowledge-Base serves as a central portal for IBEX method users.
  • It provides validation data, including negative results, for reagents.
  • Facilitates the sharing of diverse research outputs and fosters community interaction.

Conclusions:

  • The IBEX Knowledge-Base empowers method adoption and evolution.
  • It accelerates scientific discovery by reducing wasted effort and increasing data confidence.
  • Promotes a global community of researchers advancing immunofluorescence imaging techniques.