Simultaneous degradation of roxithromycin and nitrogen removal by Acinetobacter pittii TR1: Performances, pathways,...

Pharmaceutical and aquaculture wastewater contains not only antibiotics but also high concentrations of nitrogen, but few studies have been conducted on bacteria that target this complex pollution for degradation. A novel heterotrophic nitrifying aerobic denitrifying (HN-AD) strain Acinetobacter pittii TR1 isolated from soil. When the C/N ratio was 20, the strain could degrade 50 mg/L roxithromycin (ROX) and the nitrogen removal rate was 96.06% with no accumulation of nitrite. The nitrogen metabolism pathway of strain TR1 was conjectured by NH₄⁺-N → (NH₂OH) → NO₂^--N → NO₃^--N → NO₂^--N → (NO → N₂O → N₂). Nitrogen balance analysis showed that 50.43% of the initial total nitrogen (TN) was converted to gaseous nitrogen and 45.39% was assimilated by TR1. Lower concentrations of ROX affected the activity of key enzymes, and the expression of the denitrifying genes hao, napA, nirK, norZ, and nosZ were significantly up-regulated, with the gene expression of nirK being up-regulated by 15.9-fold. Cleavage of desosamine, demethylation, and phosphorylation were the main biotransformation pathways of ROX. This work offers fresh insights into the metabolic processes of HN-AD bacteria under antibiotic stress, as well as evidence supporting strain TR1 in the treatment of wastewater with nitrogen and ROX.