CtWD40-6 enhances the UV-B tolerance of safflower by regulating flavonoid accumulation

Moderate UV-B promotes plant growth, but excessive UV-B inhibits plant development. The induction mechanism of how CtWD40-6 responds to UV-B is still unclear in safflower. Our results showed that CtWD40-6 is expressed at the top of safflower leaves and is strongly induced by UV-B. To further understand the function of the CtWD40-6 gene, we overexpressed the CtWD40-6 gene in safflower or Arabidopsis. First, different transgenic materials were treated with UV-B, and we found that the survival rate of plants overexpressing CtWD40-6 was significantly higher than that of the WT type. In contrast, the survival rate of wd40-6 mutant plants was significantly decreased compared with WT type. Then DAB, NBT and Trypan Blue staining were performed on different transgenic plants before and after UV-B treatment and the results showed that the staining of mutant and WT was significantly higher than that of overexpressing CtWD40-6. By comparing the data before and after UV-B stress, we found that the flavonoid content, antioxidant enzyme activity, chlorophyll content and photosynthetic rate of transgenic plants overexpressing CtWD40-6 were higher than those of WT and mutants, thereby obtaining better UV-B tolerance. Finally, we used yeast two-hybrid and luciferase complementation experiments to prove that CtWD40-6 increases the content of safflower flavonoids by interacting with CtANS1/CtCHS1/Ct4CL1/CtFLS1, thereby enhancing the plant's UV-B tolerance. The above results provide a theoretical basis for preliminary analysis of how safflower responds to UV-B stress through the transcriptional regulation of CtWD40-6.