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Updated: Jun 3, 2025

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
Assessing the Variability of Energy Metabolisability in Barley, Rye, and Wheat Varieties for Broiler Diets.
Ibtissam Kaikat1, David Solà-Oriol1, José Francisco Pérez1
1Animal Nutrition and Welfare Service (SNiBA), Department of Animal and Food Science, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Energy metabolisability varies significantly across cereal varieties, with wheat showing the highest and rye the lowest. Enzyme supplementation benefits low-metabolisability barley varieties most.
Area of Science:
- Animal Nutrition
- Feed Science
- Agricultural Chemistry
Background:
- Energy metabolisability in animal feed is crucial for optimizing growth and efficiency.
- Variability in cereal energy content can impact animal performance and feed formulation.
- Accurate assessment of energy availability from feed ingredients is essential for livestock production.
Purpose of the Study:
- To quantify the energy metabolisability variability among different varieties of barley, rye, and wheat.
- To investigate the impact of enzyme supplementation on energy metabolisability in these cereals.
- To compare the efficacy of titanium dioxide (TiO2) and ytterbium oxide (Yb2O3) as indigestible markers.
Main Methods:
- Twelve diets were formulated using 40% cereal varieties and 60% common mixture, with and without enzyme supplementation.
- 24 experimental diets were created, including TiO2 and Yb2O3 as markers.
- 432 broilers were fed experimental diets from day 1 to 15, with excreta collected on days 20 and 25.
Main Results:
- Energy metabolisability was highest in wheat (83.3%), followed by barley (77.8%), and lowest in rye (70.6%).
- Cereal variety significantly influenced energy metabolisability in barley and wheat, exceeding predictions from NIR analyses.
- Enzyme supplementation showed a greater positive impact on barley varieties with initially lower metabolisability.
Conclusions:
- Significant energy metabolisability variation exists among and within cereal varieties, impacting feed formulation.
- Enzyme supplementation can enhance energy utilization, particularly in lower-quality cereals.
- TiO2 and Yb2O3 are reliable markers for nutrient flow estimation in broiler studies.
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