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Rapid method of MIC determinations utilizing tetrazolium reduction.

T L Johnson, B A Forbes, M O'Connor-Scarlet

    American Journal of Clinical Pathology
    |March 1, 1985
    PubMed
    Summary
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    A new rapid method using tetrazolium dye reduction accurately determines antibiotic susceptibilities in gram-negative bacteria within 4 hours, significantly reducing testing time for clinical microbiology.

    Area of Science:

    • Clinical Microbiology
    • Infectious Diseases
    • Diagnostic Technology

    Background:

    • Accurate antibiotic susceptibility testing is crucial for effective treatment of bacterial infections.
    • Traditional methods require lengthy incubation periods, delaying critical treatment decisions.
    • Gram-negative fermentative organisms are common causes of clinical infections.

    Purpose of the Study:

    • To evaluate a rapid, preliminary method for antibiotic susceptibility testing.
    • To assess the accuracy and efficiency of tetrazolium dye reduction in a microdilution system.
    • To compare results with the standard minimum inhibitory concentration (MIC) method.

    Main Methods:

    • Utilized tetrazolium dye reduction as a colorimetric indicator of bacterial growth.

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  • Incorporated the dye into the standard Micro-Media MIC microdilution testing system.
  • Tested 218 clinically isolated gram-negative fermentative organisms, comparing rapid (4-hour) and standard (15-18 hour) incubation methods.

    • Main Results:

    • Achieved an overall correlation of 93% between the rapid tetrazolium MIC method and the standard MIC method.
    • Discordant results were minimal, with 6.3% minor and 0.7% major discrepancies.
    • No very major discrepancies were observed, indicating high reliability.

    Conclusions:

    • The rapid tetrazolium MIC method provides accurate preliminary antibiotic susceptibility results.
    • This method significantly reduces incubation time from 15-18 hours to 4 hours.
    • It is a low-cost, easily implemented, and effective tool for clinical microbiology laboratories.