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Hemoglobin in Submicron Particles (HbMPs) Is Stabilized Against Oxidation
Pichayut Rerkshanandana1, Xiaotong Zhao1, Yu Xiong1
1Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
Antioxidants (Basel, Switzerland)
|January 8, 2025
Summary
Modified hemoglobin submicron particles with antioxidant enzymes (Catalase, Superoxide Dismutase) and ascorbic acid enhance hemoglobin functionality and stability against oxidation.
Area of Science:
- Biochemistry
- Materials Science
- Biotechnology
Background:
- Antioxidant enzymes Catalase (CAT) and Superoxide Dismutase (SOD) are critical cellular defenses.
- These enzymes neutralize reactive oxygen species, protecting against oxidative damage.
- Hemoglobin (Hb) and its derivatives are susceptible to oxidation, impacting their function.
Purpose of the Study:
- To fabricate and characterize hemoglobin submicron particles (HbMPs) incorporating antioxidant enzymes and ascorbic acid.
- To investigate the protective effects of modified HbMPs against oxidation under cyclic oxygenation and deoxygenation.
- To evaluate the impact of antioxidant co-immobilization on Hb functionality and stability.
Main Methods:
- Hemoglobin submicron particles (HbMPs) were fabricated using the Coprecipitation Crosslinking Dissolution (CCD) technique.
- HbMPs were modified by incorporating CAT, SOD, and ascorbic acid (Vit. C).
- Total Hb content, Oxy-Hb content, and functional Hb were determined after exposure to oxidative conditions and cyclic oxygenation/deoxygenation.
Main Results:
- Modified HbMPs demonstrated protective effects against hydrogen peroxide oxidation over 16 exposure cycles.
- Ascorbic acid significantly increased functional Hb content in both normal and enzyme-modified HbMPs.
- Co-immobilization of CAT and SOD with ascorbic acid in HbMPs (CAT-SOD-HbMPs) showed the lowest reduction in functional Hb under oxidative stress.
Conclusions:
- The CCD technique enables the fabrication of antioxidant-loaded HbMPs with enhanced stability and functionality.
- Antioxidant enzyme and ascorbic acid co-immobilization significantly improves HbMPs' resistance to oxidative damage.
- Modified HbMPs show potential for applications requiring enhanced stability and protective properties under oxidative conditions.
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