Selenium Nanoparticles Suppressed Oxidative Stress and Promoted Tenocyte Marker Expression in Tendon-Derived...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Cell Biology

Background:

• Traumatic tendon injuries often result in poor healing due to oxidative stress and inflammation.
• Selenium nanoparticles (SeNPs) possess antioxidant and anti-inflammatory properties, suggesting potential therapeutic benefits.
• The impact of SeNPs on tendon-derived stem/progenitor cells (TDSCs) and tendon healing remains largely unexplored.

Purpose of the Study:

• To investigate the effects of SeNPs on the function of hydroperoxide (H₂O₂)-stimulated TDSCs.
• To assess the potential of SeNPs in mitigating oxidative stress, inflammation, and apoptosis in TDSCs.
• To evaluate SeNPs' influence on TDSC differentiation towards tenocyte lineage.

Main Methods:

• Rat patellar TDSCs were cultured and exposed to H₂O₂ with or without SeNPs.
• Cell viability, proliferation markers, oxidative stress, inflammation, and apoptosis were assessed.
• Tenocyte marker gene expression, including Sirt1 and Nrf2, was analyzed.

Main Results:

• SeNPs significantly increased TDSC viability and proliferation markers under H₂O₂-induced oxidative stress.
• SeNPs reduced oxidative stress, inflammation, and apoptosis in stimulated TDSCs.
• SeNPs treatment elevated tenocyte marker expression and promoted TDSC differentiation towards a tenocyte lineage, likely mediated by Sirt1 and Nrf2 pathways.

Conclusions:

• SeNPs effectively protect TDSCs from oxidative stress, inflammation, and apoptosis.
• SeNPs promote TDSC proliferation and enhance tenocyte differentiation, crucial for tendon healing.
• SeNPs show promise as a novel therapeutic agent for improving tendon repair outcomes.