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Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
Published on: September 6, 2012
Polystyrene-Poly(acrylic acid) Block Copolymers for Encapsulation of Butyrylcholinesterase into Injectable
Petr A Fetin1, Ivan M Zorin1, Zukhra M Shaihutdinova2,3
1Department of Macromolecular Chemistry, Institute of Chemistry, St. Petersburg State University, 7/9 Universitetskaya nab, 199034 St. Petersburg, Russia.
Abstract:
The article is devoted to the creation of enzymatic nanoreactors based on polystyrene-block-poly(acrylic acid) (PS-b-PAA) copolymers containing bioscavengers capable of neutralizing toxic esters both in the body and in the environment. Block copolymers of different amphiphilicity, hydrophilicity and molecular weights were synthesized and characterized using gel permeation chromatography, NMR and UV spectroscopy. Polymeric nanocontainers in the absence and presence of human butyrylcholinesterase were made by film hydration and characterized by dynamic light scattering and microscopy methods. Enzyme activity was determined using the Ellman method. For the first time, factors that need to be taken into account for the creation of effective enzymatic nanoreactors based on PS-b-PAA are presented. The data obtained open up the possibility of PS-b-PAA nanoreactor use for future in vivo bioscavenger studies.
