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Updated: Jun 3, 2025

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Current Knowledge on CRISPR Strategies Against Antimicrobial-Resistant Bacteria.

Carlos de la Fuente Tagarro1,2, Diego Martín-González1,2, Andrea De Lucas1,2

  • 1Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Paseo Prado de la Magdalena 3-5, 47011 Valladolid, Spain.

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|January 8, 2025
PubMed
Summary

CRISPR/Cas systems combat antimicrobial resistance by targeting bacteria and aid antibiotic discovery from fungi and bacteria. These systems activate silent gene clusters, revealing new natural products and improving industrial strains.

Keywords:
AMRBGCsCRISPR/CasStreptomycesantibioticmultidrug-resistant bacteriananoparticlesphages

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Area of Science:

  • Microbiology
  • Biotechnology
  • Genetics

Background:

  • Antimicrobial resistance (AMR) is a growing global health threat.
  • CRISPR/Cas systems offer novel strategies to combat AMR.
  • Bacteria and fungi are crucial sources for novel antibiotic discovery.

Purpose of the Study:

  • To review CRISPR/Cas applications in treating antimicrobial resistance.
  • To explore CRISPR/Cas roles in discovering new antibiotics from natural products.
  • To highlight the utility of CRISPR/Cas in understanding microbial biosynthesis and regulation.

Main Methods:

  • CRISPR/Cas systems targeting bacterial plasmids and chromosomes.
  • Delivery of CRISPR/Cas components via nanoparticles or bacteriophages.
  • Activation of silent biosynthetic gene clusters in antibiotic-producing bacteria (e.g., Streptomyces).

Main Results:

  • CRISPR/Cas effectively sensitizes or lyses AMR bacteria.
  • CRISPR/Cas facilitates the discovery of new antibiotics from uncharacterized natural products.
  • CRISPR/Cas provides insights into metabolic regulation and biosynthetic pathways in bacteria and fungi.

Conclusions:

  • CRISPR/Cas platforms are versatile tools for tackling antimicrobial resistance.
  • CRISPR/Cas significantly contributes to natural product discovery and strain improvement.
  • Further research using CRISPR/Cas will enhance antibiotic development and production.