Genetic Mapping by 55K Single-Nucleotide Polymorphism Array Reveals Candidate Genes for Tillering Trait in Wheat...

Area of Science:

• Plant genetics and breeding
• Agronomy
• Molecular biology

Background:

• Tiller number is crucial for wheat yield potential.
• While some genes controlling tillering are known, regulatory mechanisms are unclear.

Purpose of the Study:

• To elucidate the genetic basis of the dwarf-monoculm (dmc) mutant in wheat.
• To identify genes regulating wheat tillering.

Main Methods:

• Genetic analysis of F2 populations derived from a dmc mutant crossed with multiple tiller parents.
• Mapping of tillering genes using a wheat 55K SNP array and transcriptomic data.
• Identification of candidate genes associated with tillering traits.

Main Results:

• The dmc mutant's phenotype is controlled by two dominant genes.
• Tillering genes were mapped to chromosome 5B (60-100 Mb) and 7A (135-160 Mb).
• Sixteen candidate genes were identified, including two potentially involved in auxin response and transport.

Conclusions:

• The genetic basis of the dmc mutant was elucidated.
• Two candidate genes, TraesCS5B02G058800 and TraesCS7A02G184200, are proposed as regulatory genes for wheat tillering.
• This research provides valuable reference genes for future studies on wheat tillering.