Related Experiment Video
Updated: Jun 3, 2025
07:40
Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
1.1K
Mitigating Oxidative Stress and Promoting Cellular Longevity with Mushroom Extracts
Menna-Allah E Abdelkader1,2, Hatungimana Mediatrice1,3, Dongmei Lin1
1China National Engineering Research Center of Juncao Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Foods (Basel, Switzerland)
|January 8, 2025
Summary
Medicinal mushrooms like Agaricus bisporus and Hericium erinaceus show potent antioxidant and anti-aging benefits. Extracts promote cell proliferation, DNA synthesis, and reduce oxidative stress, suggesting natural anti-aging potential.
Area of Science:
- * Mycology and Biochemistry
- * Cell Biology and Aging Research
- * Natural Product Chemistry
Background:
- * Oxidative stress, caused by free radicals, accelerates aging and contributes to health issues.
- * Medicinal and edible mushrooms are recognized for their potential health-promoting properties.
- * Understanding the antioxidant and anti-aging mechanisms of mushrooms is crucial for developing natural interventions.
Purpose of the Study:
- * To evaluate the antioxidant and anti-aging potential of four mushroom species: Ganoderma lucidum, Hericium erinaceus, Pleurotus ostreatus, and Agaricus bisporus.
- * To assess the effects of mushroom extracts on cellular processes related to aging in vitro.
- * To investigate the immunomodulatory and anti-inflammatory activities of these mushroom extracts.
Main Methods:
- * Antioxidant activity assessed using DPPH, ABTS, and reducing power assays.
- * Anti-aging effects evaluated on D-galactose-induced aged Human Skin Fibroblasts (HSF) cells.
- * Cell proliferation, cell cycle progression, DNA synthesis, DNA damage, and Reactive Oxygen Species (ROS) levels were measured.
- * Immunomodulatory effects assessed by analyzing Interleukin (IL)-2, IL-4, and IL-6 expression.
Main Results:
- * All tested mushroom extracts exhibited significant antioxidant and anti-aging properties.
- * Agaricus bisporus extract (0.03 mg/mL) promoted cell proliferation by 87%.
- * Hericium erinaceus extract (0.03 mg/mL) enhanced cell cycle progression and DNA synthesis.
- * Extracts reduced DNA damage and ROS levels, mitigating oxidative stress.
- * Mushroom extracts demonstrated immunomodulatory effects by upregulating IL-2/IL-4 and downregulating IL-6.
Conclusions:
- * Mushroom extracts possess significant antioxidant and anti-aging capabilities, offering protection against oxidative stress.
- * These natural agents can promote cellular health by enhancing proliferation, cell cycle progression, and DNA synthesis.
- * The immunomodulatory and anti-inflammatory effects further support the potential of mushrooms for overall health promotion.
- * Further research into specific bioactive compounds and in vivo efficacy is warranted.
Related Concept Videos
01:46Electron Transport Chain: Complex I and II
11.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.1K
01:37Mitochondria
10.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
10.5K
01:26Aging
36
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
36