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Updated: Jun 3, 2025

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LAP1 Interactome Profiling Provides New Insights into LAP1's Physiological Functions.

Cátia D Pereira1, Guadalupe Espadas2,3, Filipa Martins1

  • 1Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal.

International Journal of Molecular Sciences
|January 8, 2025
PubMed
Summary

Lamina-associated polypeptide 1 (LAP1) is crucial for cell health, interacting with DNA repair and RNA processing. This study maps LAP1

Keywords:
DNA repairbioinformaticsheterochromatin assemblyprotein–protein interactionsproteomicsproteostasis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genomics

Background:

  • The nuclear envelope (NE) is vital for cellular function, regulated by specialized proteins.
  • Lamina-associated polypeptide 1 (LAP1) is an NE protein with largely unknown functions, mutations of which are pathogenic.
  • Understanding LAP1's roles is critical for human health due to its essential nature.

Purpose of the Study:

  • To map the protein interactome of LAP1 to understand its diverse cellular functions.
  • To identify novel LAP1-interacting proteins and characterize their functional attributes.
  • To elucidate the multifaceted roles of LAP1 in maintaining cellular integrity and function.

Main Methods:

  • In silico analysis to predict LAP1-interacting proteins.
  • Mass spectrometry-based proteomics to identify LAP1 interactors.
  • Bioinformatics tools for functional characterization of identified proteins.
  • Validation of novel interactions with DNA repair proteins.

Main Results:

  • Identified a complex network of LAP1-interacting proteins.
  • Revealed LAP1's involvement in chromatin organization, cytoskeleton, DNA repair, RNA processing, translation, and protein turnover.
  • Confirmed novel interactions between LAP1 and DNA repair proteins.
  • Demonstrated LAP1's significant role in maintaining genomic stability.

Conclusions:

  • LAP1 is a multifunctional NE protein with a critical role in diverse cellular processes.
  • The study provides a comprehensive interactome map of LAP1, highlighting its involvement in DNA repair and genomic stability.
  • Further research into LAP1's interactome will advance understanding of its mechanistic functions and implications for human health.