Related Experiment Video
Updated: Jun 3, 2025
10:58
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
12.6K
GmbZIP4a/b Positively Regulate Nodule Number by Affecting Cytokinin Biosynthesis in Glycine max
Yongjie Meng1, Nan Wang2, Xin Wang1
1Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou 510006, China.
International Journal of Molecular Sciences
|January 8, 2025
Summary
Soybean
Area of Science:
- Plant molecular biology
- Plant-microbe interactions
- Nitrogen fixation
Background:
- Legumes form symbiotic nitrogen fixation (SNF) with rhizobia, requiring optimized nodule formation.
- TGACG BINDING FACTOR1/4 (TGA1/4) are involved in plant immune responses.
- Understanding regulators of nodule number is crucial for crop improvement.
Purpose of the Study:
- To investigate the role of GmbZIP4a and GmbZIP4b in legume nodulation.
- To determine the molecular mechanisms underlying GmbZIP4a/b-mediated regulation of nodule development.
Main Methods:
- CRISPR/Cas9 gene editing to create gmbzip4a/b double mutants.
- β-glucuronidase staining and qRT-PCR to assess gene expression.
- Nodulation assays and transcriptome analysis.
Main Results:
- GmbZIP4a and GmbZIP4b are induced by rhizobial inoculation.
- gmbzip4a/b mutants show reduced infection threads and nodule numbers.
- Cytokinin (CTK) signaling pathway genes are downregulated in mutants, indicating GmbZIP4a/b's role in CTK activation.
Conclusions:
- GmbZIP4a/b positively regulate legume nodule formation.
- These transcription factors promote nodule development by activating the CTK signaling pathway during early nodulation stages.
Related Concept Videos
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
02:14GTPases and their Regulation
8.3K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
8.3K
01:16TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K