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LncRNA-MSTRG.19083.1 Targets NTRK2 as a miR-429-y Sponge to Regulate Circadian Rhythm via the cAMP Pathway in Yak
Tianan Li1,2, Qiu Yan1,2, Jinghong Nan1,2
1College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China.
Abstract:
Long noncoding RNAs (LncRNAs) play essential roles in numerous biological processes in mammals, such as reproductive physiology and endocrinology. Cryptorchidism is a common male reproductive disease. Circadian rhythms are actively expressed in the reproductive system. In this study, a total of 191 LncRNAs were obtained from yak testes and cryptorchids. Then, we identified NTRK2's relationship to circadian rhythm and behavioral processes. Meanwhile, the ceRNA (LncRNA-MSTRG.19083.1/miR-429-y/NTRK2) network was constructed, and its influence on circadian rhythm was revealed. The results showed that NTRK2 and LncRNA-MSTRG.19083.1 were significantly upregulated, and miR-429-y was obviously decreased in cryptorchid tissue; NTRK2 protein was mainly distributed in the Leydig cells of the testis. In addition, the upregulation of the expression level of miR-429-y resulted in the significant downregulation of LncRNA and NTRK2 levels, while the mRNA and protein levels of CREB, CLOCK, and BMAL1 were significantly upregulated; the knockdown of miR-429-y resulted in the opposite changes. Our findings suggested that LncRNA-MSTRG.19083.1 competitively binds to miR-429-y to target NTRK2 to regulate circadian rhythm through the cAMP pathway. Taken together, the results of our study provide a comprehensive understanding of how the LncRNA-miRNA-mRNA networks operate when yak cryptorchidism occurs. Knowledge of circadian-rhythm-associated mRNAs and LncRNAs could be useful for better understanding the relationship between circadian rhythm and reproduction.
Insights
Long noncoding RNAs (LncRNAs) regulate circadian rhythm in yak cryptorchidism. LncRNA-MSTRG.19083.1 targets NTRK2 via miR-429-y, impacting reproductive health.
Area of Science:
- Reproductive Biology
- Molecular Endocrinology
- Chronobiology
Background:
- Long noncoding RNAs (LncRNAs) are crucial in mammalian reproductive physiology.
- Cryptorchidism is a prevalent male reproductive disorder.
- Circadian rhythms are integral to reproductive system function.
Purpose of the Study:
- To investigate the role of LncRNAs in yak cryptorchidism.
- To elucidate the relationship between NTRK2, circadian rhythm, and reproductive processes.
- To construct and analyze a ceRNA network involved in cryptorchidism.
Main Methods:
- Differential expression analysis of LncRNAs in yak testicular tissues.
- Construction of a LncRNA-miRNA-mRNA (ceRNA) network.
- Validation of molecular interactions and pathway analysis.
Main Results:
- Identified 191 LncRNAs from yak testes; NTRK2 and LncRNA-MSTRG.19083.1 were upregulated, miR-429-y downregulated in cryptorchid tissue.
- NTRK2 protein localized to Leydig cells.
- miR-429-y upregulation decreased LncRNA and NTRK2 levels, increasing CREB, CLOCK, and BMAL1; knockdown showed opposite effects.
Conclusions:
- LncRNA-MSTRG.19083.1 competitively binds miR-429-y to target NTRK2, regulating circadian rhythm via the cAMP pathway.
- This LncRNA-miRNA-mRNA network provides insights into yak cryptorchidism pathogenesis.
- Understanding circadian rhythm-associated molecules aids in studying reproduction.
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