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Updated: Jun 3, 2025
Invasion of Human Cells by a Bacterial Pathogen
Published on: March 21, 2011
Directional and Strain-Specific Interaction Between Lactobacillus plantarum and Staphylococcus aureus
Sandeep Kondakala1, Sunghyun Yoon1, Soumana Daddy Gaoh1
1Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Abstract:
The interaction between Lactobacillus plantarum and Staphylococcus aureus strains FRI-1169 and MN8, two original isolated strains from menstrual toxic shock syndrome (mTSS) cases, is a key focus for developing non-antibiotic strategies to control S. aureus-related infections. While the antagonistic effects of Lactobacilli species on S. aureus through mechanisms like organic acid and bacteriocin production are known, the molecular dynamics of these interactions remain underexplored. This study employs a proteomic approach to analyze the interactions between L. plantarum WCFS1 and S. aureus strains, FRI-1169 and MN8, during co-culture. We profiled differentially expressed proteins (DEPs) found in the spent media and cytosols of both bacteria, revealing distinct directional and strain-specific responses. The findings demonstrate that L. plantarum exerts a more pronounced effect on S. aureus, with more DEPs and upregulated proteins, while S. aureus showed fewer DEPs and more downregulated proteins. These strain-specific interactions highlight the complex metabolic and regulatory adjustments between these bacterial species. This research provides valuable insights into the molecular mechanisms of Lactobacillus-S. aureus antagonism and underscores the potential of proteomic analysis as a powerful tool for studying bacterial dynamics in co-culture systems.
Insights
Lactobacillus plantarum significantly impacts Staphylococcus aureus, altering protein expression in co-cultures. This proteomic study reveals strain-specific interactions crucial for developing non-antibiotic strategies against S. aureus infections.
Area of Science:
- Microbiology
- Proteomics
- Bacterial Interactions
Background:
- Staphylococcus aureus causes infections, including toxic shock syndrome.
- Lactobacillus species can inhibit S. aureus through organic acids and bacteriocins.
- Molecular mechanisms of Lactobacillus-S. aureus interactions require further investigation.
Purpose of the Study:
- To analyze the proteomic interactions between Lactobacillus plantarum and Staphylococcus aureus strains.
- To understand the molecular dynamics of bacterial antagonism in co-culture.
- To identify strain-specific responses during co-cultivation.
Main Methods:
- Co-cultivation of Lactobacillus plantarum WCFS1 with Staphylococcus aureus strains FRI-1169 and MN8.
- Proteomic analysis of differentially expressed proteins (DEPs) in spent media and cytosols.
- Comparison of protein expression profiles to identify bacterial responses.
Main Results:
- L. plantarum exhibited a more significant effect on S. aureus, with more DEPs and upregulated proteins.
- S. aureus showed fewer DEPs and predominantly downregulated proteins in response to L. plantarum.
- Distinct, directional, and strain-specific proteomic responses were observed.
Conclusions:
- L. plantarum exerts a strong antagonistic effect on S. aureus at the proteomic level.
- Proteomic analysis is a valuable tool for elucidating complex bacterial interactions.
- Findings support the development of non-antibiotic strategies targeting S. aureus infections.
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