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An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

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Published on: January 25, 2011

The Effect of Oral Care Product Ingredients on Oral Pathogenic Bacteria Transcriptomics Through RNA-Seq.

Ping Hu¹, Sancai Xie¹, Baochen Shi¹

¹Discovery & Innovation Platforms, The Procter & Gamble Company, Cincinnati, OH 45202, USA.

|January 8, 2025

Summary

Common oral care ingredients impact bacterial gene expression, revealing molecular mechanisms against cavities and gum disease. Stannous fluoride and cetylpyridinium chloride show strong antibacterial effects.

Keywords:

RNA-Seq bacteria cetylpyridinium chloride oral care pathogen stannous fluoride transcriptomics virulence factors

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Published on: January 13, 2016

Area of Science:

Oral microbiology
Molecular biology
Bacterial transcriptomics

Background:

Oral bacteria cause cavities, gum disease, and bad breath.
Molecular mechanisms of oral care ingredients are not fully understood.

Purpose of the Study:

Elucidate molecular mechanisms of common oral care ingredients.
Analyze gene expression profiles of oral bacteria exposed to these ingredients.

Main Methods:

High-throughput bacterial transcriptomics study.
Analyzed gene expression of six oral bacteria (Gram-positive and Gram-negative).
Exposed bacteria to nine common toothpaste/mouthwash ingredients.

Main Results:

Observed significant, reproducible ingredient-based transcriptional responses.
Demonstrated differential effects of ingredients on bacterial gene expression.
Stannous fluoride, stannous chloride, and cetylpyridinium chloride showed robust efficacy.

Conclusions:

Oral care ingredients affect oral microbiome at the molecular level.
Ingredient combinations may be more effective in complex oral environments.
Understanding these mechanisms can improve oral hygiene product development.