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Isolation and Characterization of a Lytic Phage PaTJ Against Pseudomonas aeruginosa
Jiayu Gu1,2, Xinqiao Zhang3, Tianlang Liu1
1Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, China.
Abstract:
Pseudomonas aeruginosa is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a Pseudomonas lytic phage, PaTJ, from wastewater. PaTJ belongs to the phage family Mesyanzhinovviridae, and is featured by short latency (30 min) and large burst size (103 PFU per infected cell). Our investigation revealed that PaTJ utilizes the type IV Pili (T4P) as a receptor. Transcriptome analysis of PaTJ infected host at latent stage showed distinct expression patterns of PaTJ encoding genes involved in replication and structure assembly, without expression of the majority of toxic accessory genes responsible for phage release. In addition, host bacteria exhibited specific induction of host metabolism-related genes in response to the PaTJ's infection. Furthermore, our findings demonstrated the PaTJ's potential in degrading biofilms. This work sheds light on the multifaceted impact of this lytic phage PaTJ on P. aeruginosa, presenting potential applications in both gene expression modulation and biofilm management.
Insights
This study isolated the Pseudomonas lytic phage PaTJ, which effectively targets multidrug-resistant Pseudomonas aeruginosa. PaTJ shows potential for gene expression modulation and biofilm management in treating infections.
Area of Science:
- Microbiology
- Virology
- Bacteriology
Background:
- Pseudomonas aeruginosa infections pose a significant global health challenge.
- Multidrug-resistant strains necessitate novel therapeutic strategies.
- Phage therapy offers a promising alternative to conventional antibiotics.
Purpose of the Study:
- To isolate and characterize a novel Pseudomonas lytic phage, PaTJ.
- To investigate the interaction between PaTJ and Pseudomonas aeruginosa.
- To explore the therapeutic potential of PaTJ in biofilm management and gene expression modulation.
Main Methods:
- Isolation and characterization of Pseudomonas lytic phage PaTJ from wastewater.
- Identification of type IV Pili (T4P) as the phage receptor.
- Transcriptome analysis of infected host cells during the latent stage.
- Assessment of PaTJ's efficacy in degrading Pseudomonas aeruginosa biofilms.
Main Results:
- PaTJ, a Mesyanzhinovviridae phage, exhibits short latency (30 min) and a large burst size (10^3 PFU/cell).
- Phage infection triggers specific host metabolic gene induction and modulates phage gene expression.
- PaTJ effectively degrades Pseudomonas aeruginosa biofilms.
- PaTJ utilizes type IV Pili (T4P) as its primary receptor.
Conclusions:
- PaTJ is a potent lytic phage against Pseudomonas aeruginosa.
- The phage demonstrates significant potential for controlling infections caused by multidrug-resistant strains.
- PaTJ offers novel applications in modulating gene expression and managing biofilms.
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