Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2025

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

24.0K

PD1-Targeted Transgene Delivery to Treg Cells.

Vladislav A Zhuchkov1,2, Yulia E Kravchenko1, Elena I Frolova1

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.

Viruses
|January 8, 2025
PubMed
Summary

Researchers developed targeted lentiviral vectors (LVs) to deliver gene therapy to specific immune cells. These PD-1 targeted LVs selectively target and modify T cells, offering a new approach to enhance anti-tumor immunity in cancer treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Switchable Retargeting of Lentiviral Vectors Through a VSV-G-Binding Adapter Molecule.

Viruses·2025
Same author

Donor-Acceptor (Perylenethienyl)Ethylenes as Singlet Oxygen-Photogenerating Viral Inhibitors.

Chembiochem : a European journal of chemical biology·2025
Same author

A Novel Anti-CD47 Nanobody Tetramer for Cancer Therapy.

Antibodies (Basel, Switzerland)·2024
Same author

Unwinding the SARS-CoV-2 Ribosomal Frameshifting Pseudoknot with LNA and G-Clamp-Modified Phosphorothioate Oligonucleotides Inhibits Viral Replication.

Biomolecules·2023
Same author

Alkyl Derivatives of Perylene Photosensitizing Antivirals: Towards Understanding the Influence of Lipophilicity.

International journal of molecular sciences·2023
Same author

Alphavirus-induced transcriptional and translational shutoffs play major roles in blocking the formation of stress granules.

Journal of virology·2023

Area of Science:

  • Immunology
  • Gene Therapy
  • Oncology

Background:

  • Precise targeting of lentiviral vectors (LVs) is essential for effective gene therapy, especially in cancer for modulating the tumor microenvironment.
  • Programmed cell death protein 1 (PD-1) is upregulated on tumor-infiltrating T lymphocytes, including regulatory T cells (Tregs) that suppress immune responses.

Purpose of the Study:

  • To develop PD-1 targeted LVs for selective gene delivery to PD-1 expressing cells.
  • To evaluate the efficacy of these LVs in modulating T cell responses and suppressing FOXP3 expression in Tregs.

Main Methods:

  • Engineered LVs by incorporating an anti-PD-1 nanobody (nb102c3) into modified viral glycoproteins (measles virus H and VSV-Gmut).
  • Assessed LV retargeting specificity and transduction efficiency in activated T lymphocytes.
Keywords:
FOXP3PD1Treglentivectornanobodyretargeting

More Related Videos

Generation of Human Chimeric Antigen Receptor Regulatory T Cells
10:29

Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Published on: January 3, 2025

1.2K
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

8.7K

Related Experiment Videos

Last Updated: Jun 3, 2025

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

24.0K
Generation of Human Chimeric Antigen Receptor Regulatory T Cells
10:29

Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Published on: January 3, 2025

1.2K
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

8.7K
  • Delivered shRNA via LVs to suppress FOXP3 expression in Tregs.

    • Main Results:

    • PD-1 targeted LVs demonstrated tropism towards PD-1+ cells, selectively transducing activated T lymphocytes while sparing naive T cells.
    • Suppression of FOXP3 in Tregs reduced their immune suppressive activity.
    • PD-1 targeted glycoprotein H offered higher specificity, while VSV-Gmut achieved higher viral titers but with less selectivity.

    Conclusions:

    • PD-1 targeted LVs represent a novel strategy for modulating immune responses within the tumor microenvironment.
    • This approach holds potential for developing new therapeutic strategies to enhance anti-tumor immunity.