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Respiratory Syncytial Virus Disease

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...

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Updated: Jun 13, 2026

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Published on: February 28, 2021

Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory

Victor H K Lam¹, Aleena Ghafoor¹, Yazan Khan¹

¹Department of Microbiology and Immunology, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada.

|January 8, 2025

Summary

This study details a protocol for culturing nasal epithelial cells using air-liquid interface (ALI) culture for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research. The method addresses challenges in imaging, mucus detection, and viral quantification in vitro.

Keywords:

cell culture cell isolation microbiology microscopy

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Last Updated: Jun 13, 2026

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Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Published on: September 22, 2023

Area of Science:

* Respiratory biology
* Virology
* Cell culture techniques

Background:

* Air-liquid interface (ALI) culture models airway epithelial cells in vitro.
* Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust in vitro models for study.
* Nasal turbinate tissues offer a source for primary airway epithelial cells.

Purpose of the Study:

* To present a detailed protocol for isolating and culturing human nasal epithelial cells.
* To adapt these cells for use in air-liquid interface (ALI) cultures.
* To enable SARS-CoV-2 infection studies using these primary cell models.

Main Methods:

* Isolation of nasal epithelial cells from turbinate tissues.
* Establishment of air-liquid interface (ALI) cultures.
* Optimization of imaging, mucus detection, and intracellular viral quantification techniques.

Main Results:

* Successful isolation and ALI culture of nasal epithelial cells.
* Established methods for imaging fragile ALI cultures.
* Demonstrated detection of mucus production and quantification of intracellular SARS-CoV-2.
* Provided data on optimal ALI maturation duration for experiments.

Conclusions:

* The presented protocol facilitates the generation of a functional in vitro model of the nasal airway epithelium.
* This model is suitable for investigating SARS-CoV-2 infection dynamics.
* The protocol offers flexibility for optimizing specific experimental hypotheses in respiratory virus research.