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Gingival keratinocyte adhesion on atomic layer-deposited hydroxyapatite coated titanium
Faleh Abushahba1,2, Sini Riivari2, Nagat Areid2
1Department of Biomaterials Science and Turku Clinical Biomaterials Center-TCBC, Institute of Dentistry, University of Turku, Turku, Finland.
Journal of Biomaterials Applications
|January 8, 2025
Summary
Atomic layer deposited hydroxyapatite (ALD-HA) coating enhances human gingival keratinocyte (HGK) cell adhesion, spreading, and proliferation on titanium surfaces. This ALD-HA coating shows potential for improving mucosal attachment on implant surfaces.
Area of Science:
- Biomaterials Science
- Cell Biology
- Surface Chemistry
Background:
- Titanium (Ti) is widely used in dental implants.
- Improving osseointegration and mucosal attachment is crucial for implant success.
- Hydroxyapatite (HA) coatings can enhance Ti surface biocompatibility.
Purpose of the Study:
- To evaluate the effect of atomic layer deposited hydroxyapatite (ALD-HA) coating on titanium surfaces.
- To assess HGK cell adhesion, spreading, viability, and hemidesmosome formation on ALD-HA coated Ti.
- To investigate the potential of ALD-HA for enhancing mucosal attachment on implant surfaces.
Main Methods:
- ALD-HA coating of Ti substrates.
- Surface characterization using SEM and EDS.
- Assessment of HGK cell adhesion, proliferation, and spreading.
- Evaluation of hemidesmosome formation and integrin expression via Western Blot and confocal microscopy.
Main Results:
- ALD-HA coating was confirmed on Ti surfaces.
- Significantly higher HGK cell attachment and spreading on ALD-HA coated surfaces compared to non-coated controls.
- Increased expression of laminin γ2 on ALD-HA coated surfaces.
- No significant difference in integrin α6 or β4 expression.
Conclusions:
- ALD-HA coating favorably impacts HGK cell proliferation, growth, and spreading.
- ALD-HA coating promotes hemidesmosome formation and expression of adhesion-related proteins.
- ALD-HA coating demonstrates significant potential for improving mucosal attachment on implant surfaces.
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